Plant Dehydrogenase(PDHA) Activity Assay Kit
Cat.No:BC3125 Solarbio
Detection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
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Plant Dehydrogenase(PDHA) Activity Assay KitDetection Method:Spectrophotometer/Microplate Reader
Storage:Store at 2-8℃,6 months
Qty:
Size:
Reagents Required But Not Provided | Please check the manual first, there are some reagents you should prepare by yourself. |
Name | Plant Dehydrogenase(PDHA) Activity Assay Kit |
Detection Method | Spectrophotometer/Microplate Reader |
Storage | Store at 2-8℃,6 months |
Recommend | If the 96-well plate is used, the polystyrene material is not used, and the 96-well quartz plate is recommended |
Unit | Kit |
Specification | 100T/48S |
Determination of Significance:
The activity of plant dehydrogenase (PDHA) is largely reflects the active state of the organism, which can directly indicate the ability of biological cells to degrade its matrix.
Measurement Principle:
The hydrogen acceptor 2,3,5-triphenyl tetrazolium chloride (TTC) generates red triphenylformazone (TFF) after receiving hydrogen during cell respiration. TFF has a characteristic absorption peak at 485 nm, the PDHA activity can quantified by measuring the absorbance at 485 nm.
Self Provided:
Microplate Reader or spectrophotometer, water bath, desk centrifuge, water bath, pipette, micro glass cuvette/96 well flat-bottom plate (non-polystyrene/polypropylene material), mortar/homogenizer, ethyl acetate (express delivery is not allowed), ice and distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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