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Determination of Significance：
The soluble protein content of the sample is often used to calculate the enzyme activity. In addition, soluble protein content is also used for quality analysis of food.In strong alkaline solution, biuret forms purple complex with CuSO4, the color of purple complex is dir
Measurement Principle：
In strong alkaline solution, biuret forms purple complex with CuSO4, the color of purple complex is directly proportional to protein concentration, but not related to protein molecular weight and amino acid composition, so it can be used to determine protein content. This method can be applied to the samples with high protein concentration, especially animal materials.
Self Provided：
Spectrophotometer/microplate reader, transferpettor, micro glass cuvette/96 well flat-bottom plate and distilled water.
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