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Storage:Store at -20℃，6 months
Determination of Significance：
Mitochondrial complex II is the same as succinate-Co-enzyme Q reductase, which exists widely in mitochondria of animal, plant, microorganisms and cultured cells. It catalyzes succinic acid to form fumaric acid, reduce FAD to form FADH2. The FADH2 reduce oxidized CoQ to form reduced CoQ, which is a branch of respiratory electron transport chain.
Measurement Principle：
CoQ that a catalytic product of complex II could reduce 2,6-dichloroindophenol, which has absorbance at 605 nm, the activity of enzyme can be calculated by detecting the decrease rate of 2, 6-dichlorindolepheno.
Self Provided：
Spectrophotometer/microplate reader, micro glass cuvette/96 well plate, water bath, desk centrifuge, transferpettor, acetone, mortar/homogenizer/cell ultrasonic crusher, acetone, ice and distilled water.
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