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Determination of Significance：
Granule-Bound Starch Synthase (GBSS，EC 2.4.1.21) is present in the amyloid body in a bound state, catalyzing the elongation reaction of the starch chain, and is mainly responsible for the synthesis of amylose.
Measurement Principle：
GBSS catalyzes the reaction of ADPG with starch primer (glucan), transferring glucose molecules to starch primers, and simultaneously generating ADP. Further, the pyruvate kinase, hexokinase and glucose-6-phosphate dehydrogenase added in the reaction system sequentially catalyze the reduction of NADP+ to NADPH, wherein the amount of NADPH is proportional to the amount of ADP produced by the previous reaction, and the NADPH is measured at 340 nm. Increase the amount to calculate GBSS activity.
Self Provided：
Spectrophotometer/Microplate Reader, Water Bath, Centrifuge, Transferpettor, Micro Quartz Cuvette/96 Well Flat-Bottom Plate (UV plate), Mortar, Ice and Distilled Water.
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