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Determination of Significance：
Glucose-6-phosphatase (G6Pase, EC 3.1.3.9) is a kind of phosphatase which hydrolyzes phosphate compounds. It widely exists in animals, plants, microorganisms and cells. It is a restriction enzyme which hydrolyzes glucose-6-phosphate to produce glucose in the process of gluconeogenesis. It plays an important role in maintaining the dynamic balance of blood glucose.
Measurement Principle：
G6P catalyzes glucose-6-phosphate to produce glucose and inorganic phosphorus. The increase of inorganic phosphorus content by molybdenum blue method can reflect the activity of G6P.
Self Provided：
Spectrophotometer/Microplate reader, low temperature desktop centrifuge, water bath pot, micro glass cuvette/96 well flat-bottom plate, adjustable pipette, mortar/homogenizer, EP tube, ice and distilled water.
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