Determination of Significance:
Iron is one of the essential trace elements in human body, which is the main component of hemoglobin, myoglobin, cytochrome and other enzyme systems. Iron can assist in the transport of oxygen and promote fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect the immune function of the body.
Measurement Principle:
Fe3+ is reduced by hydroxylamine hydrochloride to Fe2+. Fe2+ could react with Phenanthroline Monohydrate to form a kind of orange compound under weak acid condition, which has an absorption peak at 510 nm. According measure absorbance at 520 nm can reflect cell iron concentration.
Self Provided:
Spectrophotometer/Microplate reader, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, cell ultrasonic crusher, ice, distilled water.
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