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The PCR MasterMix produced by Solarbio contains three enzymes (Taq, Pfu, Taq plus), each corresponding to two PCR Mastermixes (dye and dye free), for a total of six PCR mastermixes.
Taq: The most efficient heat-resistant DNA polymerase with an amplification rate of about 1-2kb/min. The amplification base error rate is about 10-5. The product has A on the end and can be directly used for TA cloning.
Pfu: Currently the highest fidelity of heat-resistant DNA polymerase, base error rate is 10-6, but the amplification efficiency is lower than Taq enzyme, generally can very well amplify the fragment below 2kb. The amplification rate was about 500bp/min. The product is flat and must be added A before it can be used for TA cloning.
Taq Plus: An equal mixture of Taq and Pfu. Combined with high amplification efficiency and good fidelity. The amplification efficiency is higher than Pfu, and the fidelity is better than Taq. The amplification rate is about 1000bp/min. The product has A on the end and can be directly used for TA cloning.
The dye can be detected by electrophoresis directly after PCR MasterMix reaction. After the reaction of dye-free PCR MasterMix, the sample buffer was added for electrophoretic detection.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
