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My CartReactivity：Human,Mouse
Application：WB,ELISA,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：10721
Host：Rabbit
Qty：
Size：
| Name | Anti-POLQ Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | POLQ |
| Background | DNA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed:25642963, PubMed:25643323).MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed:25642963, PubMed:25643323).POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed:25642963). |
| Swiss Prot | O75417 |
| Gene ID | 10721 |
| Subcellular Location | Nucleus Cytoplasm |
| Immunogen | A synthetic peptide of human POLQ |
| Public Immunogen Range | 2430-2480/2590aa |
| Calculated MW | 198/290kDa |
| Observed MW | 240kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. ELISA 1:10000-100000. |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse |
| Application | WB IHC ELISA |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with POLQ antibody diluted at 1:3000;Lane: HEL,K-562,HeLa,MCF7,293T.
Immunohistochemistry of paraffin-embedded Human breast cancer with POLQ antibody diluted at 1:50.
Immunohistochemistry of paraffin-embedded Human colorectal cancer with POLQ antibody diluted at 1:50.
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