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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3030
Host：Rabbit
Qty：
Size：
| Name | Anti-HADHA Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Pig |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | HADHA |
| Background | This gene encodes the alpha subunit of the mitochondrial trifunctional protein, which catalyzes the last three steps of mitochondrial beta-oxidation of long chain fatty acids. The mitochondrial membrane-bound heterocomplex is composed of four alpha and four beta subunits, with the alpha subunit catalyzing the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities. Mutations in this gene result in trifunctional protein deficiency or LCHAD deficiency. The genes of the alpha and beta subunits of the mitochondrial trifunctional protein are located adjacent to each other in the human genome in a head-to-head orientation. |
| Swiss Prot | P40939 |
| Gene ID | 3030 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human HADHA |
| Public Immunogen Range | 414-764aa |
| Calculated MW | 83kDa |
| Observed MW | 73/75kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HADHA antibody diluted at 1:3000;Lane: HepG2,SHSY5Y,,HeLa,Jurkat,RAW264.7,NIH3T3,K562,THP1,SW480,PC3,U251,293T,MCF-7,Mouse heart,Mouse kidney,Mouse liver,Rat heart.
Immunohistochemistry of paraffin-embedded Human renal cancer with HADHA antibody diluted at 1:50.
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