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My CartReactivity：Mouse,Monkey,Human,Rat
Application：WB,IHC
Clone Type：Polyclonal Antibody
Appearance：液体
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：5347
Host：Rabbit
Qty：
Size：
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Cow;Xenopus laevis;Xenopus tropicalis |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | PLK1 |
| Background | The Ser/Thr protein kinase encoded by this gene belongs to the CDC5/Polo subfamily. It is highly expressed during mitosis and elevated levels are found in many different types of cancer. Depletion of this protein in cancer cells dramatically inhibited cell proliferation and induced apoptosis; hence, it is a target for cancer therapy. |
| Swiss Prot | P53350 |
| Gene ID | 5347 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | A synthetic peptide of human PLK1 |
| Public Immunogen Range | 485-535/603aa |
| Calculated MW | 68kDa |
| Observed MW | 63kDa |
| Purification Method | Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Appearance | 液体 |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Monkey Rat Mouse |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with PLK1 antibody diluted at 1:3000;Lane: Jurkat,Raji,Vero,K-562.
Immunohistochemistry of paraffin-embedded Human liver cancer with PLK1 antibody diluted at 1:50.
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