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My CartReactivity：Mouse,Rat,Human
Application：IP,IF,WB,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：1107
Host：Mouse
Qty：
Size：
| Name | Anti-CHD3(C-term) Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 7F6-G2-E5 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. IP 1:20. ICC 1:50-200. |
| Target | CHD3(C-term) |
| Background | This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. |
| Swiss Prot | Q12873 |
| Gene ID | 1107 |
| Subcellular Location | Cytoplasm Nucleus centrosome cytoskeleton microtubule organizing center |
| Immunogen | Recombinant protein of human CHD3(C-term) |
| Calculated MW | 223/227kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | ICC IP IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CHD3(C-term) antibody diluted at 1:1000;Lane: NTERA-2 3T3 C6 K562 HeLa.
Immunofluorescence analysis of HeLa using CHD3(C-term) antibody diluted at 1:100.
Immunoprecipitation analysis of HeLa extracts using CHD3(C-term) antibody(IP:1:20;WB:1:1000).Control IgG: Normal Mouse IgG.
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