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My CartReactivity：Rat,Human
Application：IHC,WB,IF,IP
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：8878
Host：Rabbit
Qty：
Size：
| Name | Anti-SQSTM1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IP 0.5μg-4μg. IHC 1:20-50. IF 1:20-50. |
| Target | SQSTM1 |
| Background | This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. |
| Swiss Prot | Q13501 |
| Gene ID | 8878 |
| Subcellular Location | Cytoplasm Cytoplasmic vesicle Endoplasmic reticulum Late endosome Lysosome Nucleus P-body autophagosome |
| Immunogen | Recombinant protein of human SQSTM1 |
| Public Immunogen Range | 1-440aa |
| Calculated MW | 48kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Human |
| Application | IP IF IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SQSTM1 antibody diluted at 1:1000;Lane: HeLa 293T.
Immunohistochemistry of paraffin-embedded human colon carcinoma with SQSTM1 antibody diluted at 1:100.
Immunofluorescence analysis of U2OS cells using SQSTM1 antibody diluted at 1:100.
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