Gene Name |
HMGB4 |
Synonyms |
HMGB4;dJ1007G16.5;high mobility group box 4 |
English Name |
Recombinant human HMGB4 protein |
Full Name |
high-mobility group box 4 |
Chinese Name |
高迁移率B蛋白组4 |
Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Purity |
≥85% by SDS-PAGE. |
Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year. |
Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
Concentration |
1mg/ml by SDS-PAGE. |
Swiss Prot |
Q8WW32 |
Gene ID |
127540 |
Reactivity |
Human Mouse (Bovine A.thaliana) |
Molecular Weight |
22kDa |
Interval Range |
1-186aa |
Background |
High mobility group protein B4 is a transcription factor that in humans is encoded by the HMGB4 gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues.HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor.During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole.HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus.These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus. |
Application |
Immunology research |
Source |
E.coli |
Purification Method |
NI-NTA affinity purification |
Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
Endotoxin |
Not measured |
Biological Activity |
N0 |
Lable Information |
With a 6×His tag at the N/C-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |