Gene Name |
SFRS1 |
Synonyms |
ASF;SF2;SF2p33;SFRS1;SRp30a |
English Name |
Recombinant human SFRS1 protein |
Full Name |
splicing factor,arginine/serine-rich 1 |
Chinese Name |
剪接因子,精氨酸/丝氨酸丰富1 |
Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Purity |
≥85% by SDS-PAGE. |
Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year. |
Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
Concentration |
1mg/ml by SDS-PAGE. |
Swiss Prot |
Q07955 |
Gene ID |
6426 |
Reactivity |
Human Mouse (Bovine Pig Gorilla Chicken) |
Molecular Weight |
28kDa |
Interval Range |
1-248aa |
Background |
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing.Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA.Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC.Binds preferentially to the 5'-CGAGGCG-3' motif in vitro.Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing.Isoform ASF-2 and isoform ASF-3 act as splicing repressors.May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway. |
Application |
Immunology research |
Source |
E.coli |
Purification Method |
NI-NTA affinity purification |
Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
Endotoxin |
Not measured |
Biological Activity |
N0 |
Lable Information |
With a 6×His tag at the N/C-terminus. |
Unit |
Piece |
Specification |
10ug 50ug 100ug 1mg |