| Gene Name |
TYR |
| Synonyms |
Tyr-DNA phosphodiesterase 2;hTDP2;5'-Tyr-DNA phosphodiesterase;EAPII;EAP2;TTRAP;AD-022 |
| English Name |
Recombinant human TYR protein |
| Full Name |
tyrosinase (oculocutaneous albinism IA) |
| Chinese Name |
酪氨酸酶(眼皮肤白化病) |
| Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
| Purity |
≥85% by SDS-PAGE. |
| Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
| Concentration |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
P14679 |
| Gene ID |
7299 |
| Reactivity |
Human Mouse (Chicken Bovine) |
| Molecular Weight |
41kDa |
| Interval Range |
1-377aa |
| Background |
DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate.Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue.The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation.Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2.Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals.Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate.Acts as a regulator of ribosome biogenesis following stress.Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1.Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells.Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation.Involved in non-canonical TGF-beta induced signaling routes.May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation.(Microbial infection) Also acts as a 5'-tyrosyl-RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. |
| Application |
Immunology research |
| Source |
E.coli |
| Purification Method |
NI-NTA affinity purification |
| Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| Endotoxin |
Not measured |
| Biological Activity |
N0 |
| Lable Information |
With a 6×His tag at the N/C-terminus. |
| Unit |
Piece |
| Specification |
10ug 50ug 100ug 1mg |
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