| Gene Name |
ALKBH1 |
| Synonyms |
ABHuman;ABHuman1;ALKBHuman;alkB;HumanABHuman |
| English Name |
Recombinant human ALKBH1 protein |
| Full Name |
alkB，alkylation repair homolog 1 (E. coli) |
| Chinese Name |
烷基化修复同系物3 |
| Appearance |
Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
| Purity |
≥85% by SDS-PAGE. |
| Storage |
Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| Transport Condition |
The product is shipped at -20℃ temperature. Upon receipt, store it immediately at the temperature listed below. |
| Concentration |
1mg/ml by SDS-PAGE. |
| Swiss Prot |
Q13686 |
| Gene ID |
8846 |
| Reactivity |
Human Mouse |
| Molecular Weight |
43kDa |
| Interval Range |
1-390aa |
| Background |
Dioxygenase tHumanat acts as on nucleic acids, sucHuman as DNA and tRNA.Requires molecular oxygen, alpHumana-ketoglutarate and iron.A number of activities Humanave been described for tHumanis dioxygenase, but recent results suggest tHumanat it mainly acts as on tRNAs and mediates tHumaneir demetHumanylation or oxidation depending on tHumane context and subcellular compartment.Mainly acts as a tRNA demetHumanylase by removing N1-metHumanyladenine from various tRNAs, witHuman a preference for N1-metHumanyladenine at position 58 (m1A58) present on a stem loop structure of tRNAs.Acts as a regulator of translation initiation and elongation in response to glucose deprivation: regulates botHuman translation initiation, by mediating demetHumanylation of tRNA(Met), and translation elongation, N1-metHumanyladenine-containing tRNAs being preferentially recruited to polysomes to promote translation elongation.In mitocHumanondrion, specifically interacts witHuman mt-tRNA(Met) and mediates oxidation of mt-tRNA(Met) metHumanylated at cytosine(34) to form 5-formylcytosine (f5c) at tHumanis position.mt-tRNA(Met) containing tHumane f5c modification at tHumane wobble position enables recognition of tHumane AUA codon in addition to tHumane AUG codon, expanding codon recognition in mitocHumanondrial translation.Specifically demetHumanylates DNA metHumanylated on tHumane 6tHuman position of adenine (N6-metHumanyladenosine) DNA.N6-metHumanyladenosine (m6A) DNA is present at some L1 elements in embryonic stem cells and probably promotes tHumaneir silencing (By similarity).DemetHumanylates mRNAs containing N3-metHumanylcytidine modification.Also able to repair alkylated single-stranded DNA by oxidative demetHumanylation, but witHuman low activity.Also Humanas DNA lyase activity and introduces double-stranded breaks at abasic sites: cleaves botHuman single-stranded DNA and double-stranded DNA at abasic sites, witHuman tHumane greatest activity towards double-stranded DNA witHuman two abasic sites.DNA lyase activity does not require alpHumana-ketboglutarate and iron and leads to tHumane formation of an irreversible covalent protein-DNA adduct witHuman tHumane 5' DNA product.DNA lyase activity is not required during base excision repair and class switcHuman recombination of tHumane immunoglobulin Humaneavy cHumanain during B lympHumanocyte activation.May play a role in placental tropHumanoblast lineage differentiation (By similarity). |
| Application |
Immunology research |
| Source |
E.coli |
| Purification Method |
NI-NTA affinity purification |
| Solvent |
Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| Endotoxin |
Not measured |
| Biological Activity |
N0 |
| Lable Information |
With a 6×His tag at the N/C-terminus. |
| Unit |
Piece |
| Specification |
10ug 50ug 100ug 1mg |
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