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My CartReactivity：Human,Mouse,Rat
Application：IHC,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：3155
Host：Rabbit
Qty：
Size：
| Name | Anti-HMGCL Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Predicted Reactivity | Bovine;Monkey;Orangutan |
| Dilution Ratio | WB 1:2000-5000. IHC 1:25-100. |
| Target | HMGCL |
| Background | The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Swiss Prot | P35914 |
| Gene ID | 3155 |
| Subcellular Location | Cytoplasm |
| Immunogen | Recombinant protein of human HMGCL |
| Public Immunogen Range | 1-326aa |
| Calculated MW | 20/27/34kDa |
| Observed MW | 34kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 20ul 50ul 100ul |
| Reactivity | Human Mouse Rat |
| Application | WB IHC |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with HMGCL antibody diluted at 1:2000;Lane: HepG2,Raji,Liver cancer,Mouse liver,Rat liver.
Immunohistochemistry of paraffin-embedded Human thyroid cancer with HMGCL antibody diluted at 1:50.
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