LAMP是一种新型核酸扩增技术,采用4或6条能够识别目的基因上的6个特异区域的引物,依赖于Bst DNA聚合酶的强链置换活性,在30~60分钟内DNA扩增可达109~1010倍。LAMP检测方法较多,包括染料法、浊度法、电泳法及TaqMan荧光探针法。
钙黄绿素指示剂的原理为:在核酸扩增过程中会形成大量的焦磷酸根离子,焦磷酸根离子能解开钙黄绿素与金属离子的结合,使荧光颜色从橙红色变成黄绿色,进而指示扩增情况。
本产品含钙黄绿素和锰离子,可应用于恒温扩增反应的荧光检测。
Remark:
These protocols are for reference only. Solarbio does not independently validate these
methods.
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